thermo核酸定量Qubit microRNA Assay Kit

thermo核酸定量Qubit microRNA Assay Kit

貨號 單位規(guī)格     單價 (CNY)

Q32880 100 assays   1,483.00

Q32881 500 assays   4,537.00

Sample Type (General): FFPE & Fixed Samples, RNA

For Use With (Equipment): Qubit® Fluorometer

Product Line: Qubit™

Product Size: 100 assays

Sample Type (Specific): RNA (<40 bases), Single-Stranded RNA, Single-Stranded RNA (ssRNA), Tissue (FFPE), miRNA

內(nèi)容及儲存

Store in refrigerator (2–8°C) and protect from light.

The Qubit microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA) using the Qubit Fluorometer, even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng in the assay tube following the supplied protocol below, even in the presence of mRNA.

Features of Qubit microRNA assay:

• Accurately detects as little as 0.5 ng miRNA in the assay tube

• Core dynamic range of 5–500 ng/mL

• Accurate results for sample concentrations from 50 ng/mL to 100 µg/mL

The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. Because the assay tolerates 1–20 µL of sample in an assay volume of 200 µL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100 µg/mL.

Which product to choose for microRNA quantitation?

• For 1–20 samples: use this Qubit microRNA Assay Kit with the Qubit Fluorometer

• For 20–2000 samples: use the Quant-iT microRNA Assay Kit with microplate reader

• For >2000 samples: use the Quant-iT microRNA Reagent with microplate reader

Notes:

1. The Qubit microRNA assay file is included on all Qubit 3 and Qubit 4 fluorometers, but may require installation onto the Qubit 2.0 Fluorometer. Uploading new assays to the Qubit 2.0 Fluorometer requires version 3.10 or later of the Qubit firmware.

2. 500 µL thin-walled PCR tubes are required but not included.

用于Qubit熒光計的Qubit試劑盒，配合簡單的混合讀取模式，均采用相同的操作方式。其中DNA和RNA分析試劑盒僅需孵育2分鐘，而蛋白分析試劑盒需要15分鐘 。 Qubit 4熒光計自動保存您的數(shù)據(jù)，您可用USB數(shù)據(jù)線或U盤導(dǎo)出。 除了常規(guī)的 dsDNA，寡核苷酸，總RNA，小RNA和蛋白質(zhì)的分析試劑盒，在 Ion Personal Genome Machine(PGM) 測序儀上進行測序之前，可使用專門的Ion Sphere Quality Control Kit在Qubit 4熒光計上進行Ion Sphere顆粒質(zhì)量評估。